The Journal of Heart and Lung Transplantation
Volume 27, Issue 5 , Pages 554-560 , May 2008

Efficient and Durable Gene Transfer to Transplanted Heart Using Adeno-associated Virus 9 Vector

  • Naoto Miyagi, MD

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
  • ,
  • Vinay P. Rao, MD

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
  • ,
  • Davide Ricci, MD

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
  • ,
  • Zeji Du, MD, PhD

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
  • ,
  • Guerard W. Byrne, PhD

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
  • ,
  • Kent R. Bailey, PhD

      Affiliations

    • Department of Biostatistics, Mayo Clinic, Rochester, Minnesota
  • ,
  • Hiroyuki Nakai, MD, PhD

      Affiliations

    • Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • ,
  • Stephen J. Russell, MD, PhD

      Affiliations

    • Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota.
  • ,
  • Christopher G.A. McGregor, MB, FRCS, MD (Hons)

      Affiliations

    • William J. von Liebig Transplant Center, Mayo Clinic, Rochester, Minnesota
    • Corresponding Author InformationReprint requests: Christopher G. A. McGregor, MD (Hons), William J. von Liebig Transplant Center, Mayo Clinic College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Telephone: 507-255-6038. Fax: 507-255-4500.

Received 28 September 2007 ,Revised 14 January 2008 ,Accepted 24 January 2008.

References 

  1. Wang J, Ying M, Knechtle SJ. Adenovirus-mediated gene transfer into rat cardiac allografts: comparison of direct injection and perfusion. Transplantation. 1996;61:1726–1729
  2. Ardehali A, Fyfe A, Laks H, et al. Direct gene transfer into donor hearts at the time of harvest. J Thorac Cardiovasc Surg. 1995;109:716–719
  3. Pellegrini C, O'Brien T, Yap J, Jeppsson A, Tazelaar HD, McGregor CGA. Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart. Transplant Int. 1998;11:373–377
  4. Kypson AP, Peppel K, Akhter SA, et al. Ex vivo adenovirus-mediated gene transfer to the adult rat heart. J Thorac Cardiovasc Surg. 1998;115:623–630
  5. DeBruyne LA, Li K, Chan SY, Qin L, Bishop DK, Bromberg JS. Lipid-mediated gene transfer of viral IL-10 prolongs vascularized cardiac allograft survival by inhibiting donor-specific cellular and humoral immune responses. Gene Ther. 1998;5:1079–1087
  6. Pellegrini C, Jeppsson A, Taner CB, et al. Highly efficient ex vivo gene transfer to the transplanted heart by means of hypothermic perfusion with a low dose of adenoviral vector. J Thorac Cardiovasc Surg. 2000;119:493–500
  7. Oi K, Davies WR, Tazelaar HD, et al. Ex vivo hypothermic recirculatory adenoviral gene transfer to the transplanted pig heart. J Gene Med. 2006;8:795–803
  8. Marshall E. Gene therapy death prompts review of adenovirus vector. Science. 1999;286:2244–2245
  9. Wang Z, Zhu T, Qiao C, et al. Adeno-associated virus serotype 8 efficiently delivers genes to muscle and heart. Nat Biotechnol. 2005;23:321–328
  10. Nakai H, Fuess S, Storm TA, Muramatsu S, Nara Y, Kay MA. Unrestricted hepatocyte transduction with adeno-associated virus serotype 8 vectors in mice. J Virol. 2005;79:214–224
  11. Inagaki K, Fuess S, Storm TA, et al. Robust systemic transduction with AAV9 vectors in mice: efficient global cardiac gene transfer superior to that of AAV8. Mol Ther. 2006;14:45–53
  12. Sarkar R, Mucci M, Addya S, et al. Long-term efficacy of adeno-associated virus serotypes 8 and 9 in hemophilia a dogs and mice. Hum Gene Ther. 2006;17:427–439
  13. Pacak CA, Mah CS, Thattaliyath BD, et al. Recombinant adeno-associated virus serotype 9 leads to preferential cardiac transduction in vivo. Circ Res. 2006;99:e3–e9
  14. Ono K, Lindsey ES. Improved technique of heart transplantation in rats. J Thorac Cardiovasc Surg. 1969;57:225–229
  15. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acids Res. 2001;29:e45
  16. Taylor DO, Edwards LB, Boucek MM, et al. Registry of the International Society for Heart and Lung Transplantation: twenty-second official adult heart transplant report—2005. J Heart Lung Transplant. 2005;24:945–955
  17. Isobe M, Kosuge H, Koga N, Futamatsu H, Suzuki J. Gene therapy for heart transplantation-associated acute rejection, ischemia/reperfusion injury and coronary arteriosclerosis. Curr Gene Ther. 2004;4:145–152
  18. Brauner R, Nonoyama M, Laks H, et al. Intracoronary adenovirus-mediated transfer of immunosuppressive cytokine genes prolongs allograft survival. J Thorac Cardiovasc Surg. 1997;114:923–933
  19. Kita Y, Li XK, Ohba M, et al. Prolonged cardiac allograft survival in rats systemically injected adenoviral vectors containing the CTLA4Ig gene. Transplantation. 1999;68:758–766
  20. Li J, Simeoni E, Fleury S, et al. Gene transfer of soluble interleukin-17 receptor prolongs cardiac allograft survival in a rat model. Eur J Cardiothorac Surg. 2006;29:779–783
  21. Pätilä T, Ikonen T, Rutanen J, et al. Vascular endothelial growth factor C–induced collateral formation in a model of myocardial ischemia. J Heart Lung Transplant. 2006;25:206–213
  22. Woo YJ, Zhang JC, Taylor MD, et al. One year transgene expression with adeno-associated virus cardiac gene transfer. Int J Cardiol. 2005;100:421–426
  23. Pellegrini C, O'Brien T, Jeppsson A, et al. Influence of temperature on adenovirus-mediated gene transfer. Eur J Cardiothorac Surg. 1998;13:599–603
  24. McLaren AJ, Friend PJ. Trends in organ preservation. Transplant Int. 2003;16:701–708
  25. Chen Z, Lu L, Li J, Xiao X, Fung JJ, Qian S. Prolonged survival of heart allografts transduced with AAV-CTLA4Ig. Microsurgery. 2003;23:489–493
  26. Li J, Wang D, Qian S, Chen Z, Zhu T, Xiao X. Efficient and long-term intracardiac gene transfer in delta-sarcoglycan-deficiency hamster by adeno-associated virus-2 vectors. Gene Ther. 2003;10:1807–1813
  27. Su LT, Gopal K, Wang Z, et al. Uniform scale-independent gene transfer to striated muscle after transvenular extravasation of vector. Circulation. 2005;112:1780–1788
  28. Manno CS, Arruda VR, Pierce GF, et al. Successful transduction of liver in hemophilia by AAV-Factor IX and limitations imposed by the host immune response. Nat Med. 2006;12:342–347

 Supported by the National Institutes of Health (Grant HL66958 P2) and the Mayo Clinic and Foundation, Rochester, Minnesota.

PII: S1053-2498(08)00106-X

doi: 10.1016/j.healun.2008.01.025

The Journal of Heart and Lung Transplantation
Volume 27, Issue 5 , Pages 554-560 , May 2008